Chester FC will make the short journey to Witton Albion in the FA Trophy first round on Saturday (3pm kick-off).
Witton set up the all-Cheshire clash by winning their twice rearranged third round qualifying tie at home to higher-division Boston United last night.
Vanarama National League North side Boston raced into a two-goal inside the first six minutes.
But Witton, who are pushing for promotion in the Evo-Stik Northern Premier League First Division South, roared back to win 4-2.
Among their scorers was captain Rob Hopley, the former Chester striker who netted the club's first competitive goal following its reformation .
Albion are managed by ex-Vauxhall Motors manager Carl Macauley, who spent a brief spell as the Blues' assistant manager in the second half of the 2013-14 season .
Chester have fond memories of Witton's Wincham Park.
On their last league trip to the ground, in October 2010, they won a dramatic match 3-2 thanks to an injury-time winner from George Horan .
They also claimed the Cheshire Senior Cup crown at Albion's home in April 2013 when they beat Stalybridge Celtic 2-1 in the final .