A planning application has been submitted for two wind turbines close to the Wirral Way.
The University of Liverpool has applied to install two turbines on land between Cuckoo Lane and Woodfall Lane in Little Neston.
The structures would be sited at the university’s veterinary surgery Leahurst, and also includes a substation and control units.
Wirral Way, which stretches from West Kirby to Hooton, is popular with dog walkers, families, cyclists and horse riders.
Protesters are worried the turbines would be too close to houses and Woodfall Primary School, as well as being an intrusion on green belt land, destroying wildlife and disrupting local bird populations.
They feared a fresh application. It follows a previous bid which the university withdrew 12 months ago to carry out further surveys requested by Cheshire West and Chester Council (CWaC).
The proposal had over 300 objections at the time.
A wildlife study took place last winter – which has attracted criticism about the period it was conducted.
Neil Heather, chair of Ness Conservation Society, says it will open the way to further development on the green belt land if the proposal goes ahead.
He also fears the blades could stand 350feet above sea level – endangering birds, especially the migration route from the Dee estuary.
Neil said: “The university carried out a wildlife survey, but it was only a short one conducted over the coldest winter months where wildlife is the least active.
“They also held a presentation in Neston library about the plans, and the bulk of locals who attended objected to it.
“Feelings in the community are strongly against the plan.”