Philip Whyte, 49, Simon Stafford-Smith, 37, and Max Wiesner, 29, all pleaded not guilty at Chester Magistrates Court to attacking Mark Williams on October 23.
Mr Williams was trying to gain entry to the Duttons Lane site when two incidents involving the men are alleged to have happened.
At the time it was claimed the bailiff flopped to the ground after being pushed from a gate.
Watch the video showing the confrontation below
He had attended alongside seven police cars and a police dog team to issue warning notices to those living at the camp.
Activists claimed Mr Williams had refused to provide identification while trying to get inside.
Video shot by a supporter of the campaign and police body camera footage could be used in the trial.
Whyte, Wiesner and Stafford-Smith appeared in court on December 30.
Stafford-Smith appeared by link from HMP Altcourse after being jailed over the festive period for violating his probation order.
All three men were bailed in relation to the assault allegations. A trial date has been set for March 29.
Protesters were ordered to leave Duttons Lane by December 4 after a court battle with energy company IGas, who now own the site.
Famous faces such as Green Party leader Natalie Bennett, fashion designer Vivienne Westwood and Bez of the Happy Mondays have visited the anti-frackers to show their support.
Campaigners want to stop IGas from drilling an exploratory borehole on the site because of fears over its environmental impact.