The Chester rower will race with his London 2012 silver medal winning crew-mate Peter Chambers in a lightweight four which includes Jono Clegg and Mark Aldred.
Former King’s School pupil Bartley missed out on Olympic gold by less than a second during a dramatic final four years ago.
An ever-present in the four since 2009 and World Champion in 2010, the 32-year-old is one of the most experienced rowers in the squad that will compete in Brazil.
Bartley said: "I’m really proud to be announced as part of a strong Team GB rowing squad for my first away Games.
"I’m very much looking forward to seeing what we can do as part of Team GB in the next two months and especially at the end of our journey at the Lagoa in Rio."
Bartley from Farndon helped the lightweight four win silver at the European Rowing Championships last month in Brandenburg, Germany.
Sir David Tanner, British Rowing performance director and Team GB rowing team leader, has targeted six medals at Rio 2016, three below the nine they won at London 2012.
He said: "We have had a few curved balls this season already with illness and have a final run-out at the world cup in Poznan next week before going to Rio.
"I am sure these will be a challenging but exceptional Games with a truly brilliant field of play for rowing at the Lagoa."