Parkgate Road into Chester has now reopened after a crash.
Cheshire Police had closed the road following the incident between a white Fiat Punto and a HGV at the junction with Long Lane at about 5.46am on Monday.
Officers removed blocks from the A494 through to the Blacon roundabout shortly after 11am.
A 25-year-old man and a 15-year-old boy who were both in the car were taken to hospital, the lorry driver was unharmed.
Motorists had faced rush hour delays as they were diverted around the road closure, but all built-up congestion has now eased.
Anyone with any information in relation to the incident is asked to contact Cheshire Police on 101 quoting incident number 89 of 14/3/2016.